complex scenario test (cst) Search Results


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BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) <t>Immunofluorescence</t> staining of RAW264.7 cells. (C)The protein level of <t>CD86,</t> detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.
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BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) <t>Immunofluorescence</t> staining of RAW264.7 cells. (C)The protein level of <t>CD86,</t> detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.
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BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) <t>Immunofluorescence</t> staining of RAW264.7 cells. (C)The protein level of <t>CD86,</t> detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.
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BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) <t>Immunofluorescence</t> staining of RAW264.7 cells. (C)The protein level of <t>CD86,</t> detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.
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complex scenario test (cst)  (Cell Signaling Technology Inc)
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BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) <t>Immunofluorescence</t> staining of RAW264.7 cells. (C)The protein level of <t>CD86,</t> detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.
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BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) <t>Immunofluorescence</t> staining of RAW264.7 cells. (C)The protein level of <t>CD86,</t> detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.
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Image Search Results


BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) Immunofluorescence staining of RAW264.7 cells. (C)The protein level of CD86, detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.

Journal: Heliyon

Article Title: BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway

doi: 10.1016/j.heliyon.2023.e15583

Figure Lengend Snippet: BSHXD inhibited LPS-induced M1 polarization of RAW264.7 macrophages. (A) The morphology of RAW264.7 cells observed by light microscope. (B) Immunofluorescence staining of RAW264.7 cells. (C)The protein level of CD86, detected by Western blot analysis. (D) CD86 was normalized to β-Actin and expressed in arbitrary units. (E) The proportion of F4/80+ and CD86 + in RAW264.7 cells, detected by flow cytometry. N = 3, data are expressed as mean ± SD. *p < 0.05, **p < 0.01 compared to LPS group.

Article Snippet: We also completed immunofluorescence staining (IF) for CD86 (1:200, #19589, CST) and F4/80 (1:200, #70076, CST).

Techniques: Light Microscopy, Immunofluorescence, Staining, Western Blot, Flow Cytometry

BSHXD inhibits inflammatory factors and M1 macrophages through activating AMPK. (A) The CD86 protein level, detected by Western blot analysis. (B) CD86 was normalized to β-Actin and expressed in arbitrary units. (C) IL-1β, IL-6 and TNF-α protein levels, detected by Western blot analysis. (D) IL-1β, (E) IL-6 and (F) TNF-α were normalized to β-Actin and expressed in arbitrary units. (G) The proportion of F4/80 + and CD86 + in RAW264.7 cells, detected by flow cytometry. (H) Typical images of immunofluorescence staining: red (CD86), green (F4/80), and blue (DAPI). Scale bar indicates 100 μm.N = 3. Data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01.

Journal: Heliyon

Article Title: BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway

doi: 10.1016/j.heliyon.2023.e15583

Figure Lengend Snippet: BSHXD inhibits inflammatory factors and M1 macrophages through activating AMPK. (A) The CD86 protein level, detected by Western blot analysis. (B) CD86 was normalized to β-Actin and expressed in arbitrary units. (C) IL-1β, IL-6 and TNF-α protein levels, detected by Western blot analysis. (D) IL-1β, (E) IL-6 and (F) TNF-α were normalized to β-Actin and expressed in arbitrary units. (G) The proportion of F4/80 + and CD86 + in RAW264.7 cells, detected by flow cytometry. (H) Typical images of immunofluorescence staining: red (CD86), green (F4/80), and blue (DAPI). Scale bar indicates 100 μm.N = 3. Data are presented as mean ± SD of three independent experiments. *p < 0.05, **p < 0.01.

Article Snippet: We also completed immunofluorescence staining (IF) for CD86 (1:200, #19589, CST) and F4/80 (1:200, #70076, CST).

Techniques: Western Blot, Flow Cytometry, Immunofluorescence, Staining

BSHXD inhibited LPS-induced inflammatory bone loss. (A)Immunohistochemical staining of TNF-α in mouse skull. Scale bar indicates 200 and 50 μm. (B)Immunofluorescence staining in mouse skull: red (CD86), green (F4/80), and blue (DAPI). Scale bar indicates 100 μm. (C) Number of TNF-α positive cells. (D) Number of CD86 positive cells. N = 3. All data were expressed as the mean ± SD. *p < 0.05, **p < 0.01, compared with the vehicle group.

Journal: Heliyon

Article Title: BushenHuoxue decoction suppresses M1 macrophage polarization and prevents LPS induced inflammatory bone loss by activating AMPK pathway

doi: 10.1016/j.heliyon.2023.e15583

Figure Lengend Snippet: BSHXD inhibited LPS-induced inflammatory bone loss. (A)Immunohistochemical staining of TNF-α in mouse skull. Scale bar indicates 200 and 50 μm. (B)Immunofluorescence staining in mouse skull: red (CD86), green (F4/80), and blue (DAPI). Scale bar indicates 100 μm. (C) Number of TNF-α positive cells. (D) Number of CD86 positive cells. N = 3. All data were expressed as the mean ± SD. *p < 0.05, **p < 0.01, compared with the vehicle group.

Article Snippet: We also completed immunofluorescence staining (IF) for CD86 (1:200, #19589, CST) and F4/80 (1:200, #70076, CST).

Techniques: Immunohistochemical staining, Staining, Immunofluorescence